The global NGS-Based RNA-Sequencing Market had a robust growth in 2020 and is expected to continue registering steady revenue growth in the forecast period. The market is being driven by the popularity and adoption of targeted RNA sequencing in various sectors such as research institutes, hospitals and clinics, biotechnology companies, and diagnostic labs. Targeted RNA sequencing is a precise method that helps to identify and sequence individual RNA transcripts, providing both quantitative and qualitative data. The exome sequencing segment is anticipated to have the highest revenue share, while research institutes are expected to lead in terms of application type.

Revenue growth is being fueled by advancements in sequencing technology, increasing acceptance of NGS technology, and partnerships and collaborations among major companies. However, several factors are expected to hinder market growth, including the sudden emergence of the COVID-19 pandemic, scarcity of experienced personnel, issues with storing sequencing data, and high installation and maintenance costs of machines. Despite these challenges, new business opportunities are expected to arise for major players and new entrants in the market due to the identification of known and unknown fusion gene pairs and government initiatives in population sequencing in emerging economies such as China and India.

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Several factors are driving the growth of the NGS-Based RNA-Sequencing Market, including the growing demand for personalized medicine, increasing prevalence of genetic diseases, advancements in next-generation sequencing technologies, growing focus on cancer research, and increasing investment in genomics research. Targeted RNA sequencing is a precise and accurate approach that can help in the diagnosis of genetic diseases, provide insights into their molecular mechanisms, and enable the detection of disease-associated genetic variations and biomarkers, making it a preferred option for personalized medicine.

The development of more precise and efficient RNA sequencing methods, driven by advancements in NGS technologies such as improvements in sequencing accuracy and throughput, has further fueled the growth of the market. The growing focus on cancer research and the need for better diagnostic and treatment options, along with heavy investment by governments and private organizations in genomics research, are also expected to boost the growth of the NGS-Based RNA-Sequencing Market.

The targeted RNA sequencing market has witnessed significant innovations in recent years, including single-cell targeted RNA sequencing, digital droplet PCR, fusion gene detection, long-read sequencing, and in situ sequencing. Single-cell RNA sequencing has been widely used to analyze gene expression at the individual cell level, but traditional methods can suffer from low coverage and high levels of technical noise. Targeted RNA sequencing has been applied to single-cell analysis to improve coverage of specific genes or transcripts of interest while reducing technical noise. Digital droplet PCR involves partitioning a sample into thousands of droplets, each containing one or zero copies of a target molecule, and can be used with targeted RNA sequencing to accurately measure the expression levels of specific genes or transcripts. Targeted RNA sequencing can also be used to detect fusion genes, which play a role in cancer development and serve as biomarkers for certain types of cancer.

    Long-read sequencing technologies, such as PacBio and Oxford Nanopore, have improved the accuracy and completeness of RNA sequencing data, and can be combined with targeted RNA sequencing to obtain more comprehensive coverage of transcripts of interest. In situ sequencing involves labeling RNA probes with fluorescent dyes and imaging the probes using microscopy, and can be used with targeted RNA sequencing to spatially map gene expression in tissues by designing probes for specific genes or transcripts of interest.

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